Micropropagation of Phyllanthus Amarus Schum. & Thom. by Meristem Culture
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چکیده
A novel protocol for production of multiple shoot tip clumps of Keelar nelli (Phyllanthus amarus Schum. & Thom.) were developed in vitro from shoot tip explants cultured on Murashige and Skoog (MS) medium containing only BAP or BAP with NAA, 3% sucrose and 0.8% agar. The addition of the following combination of growth substances 0.1mgLBAP and 0.05mgLNAA, caused an extensive proliferation of multiple shoot primordia. Subculture of these on the same medium was successful for the multiplication. High frequency rooting was observed in MS medium containing IBA (1.0mgL). The plants were successfully transplanted to glasshouse and grown to maturity with a survival rate of 95%.
منابع مشابه
High frequency in vitro propagation of Phyllanthus amarus Schum. & Thom. by shoot tip culture.
With the aim of micropropagation of Phyllanthus amarus, an important medicinal herb, shoot tips were cultured in Murashige and Skoog's medium supplemented with kinetin/ BAP singly or in combination with IAA. Growth regulators at lower range (0.1-1.0 mg L(-1)) stimulated direct regeneration of shoots. Kinetin was superior to BAP and kinetin-IAA combination was more suitable than kinetin alone. A...
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تاریخ انتشار 2006